<p><strong><span data-contrast="auto">What if… you could pioneer a new approach to designing life’s most essential molecules?</span></strong><span data-ccp-props="{"335559739":200}"> </span></p> <p><span data-contrast="auto">FL116, backed by Flagship Pioneering, is an early-stage biotechnology company transforming the way biomolecules are synthesized. Our innovative platform has the potential to redefine how critical research, diagnostic, and therapeutic molecules are designed and built. We are seeking passionate problem solvers ready to make a profound impact on biotechnology and therapeutic innovation.</span><span data-ccp-props="{"335559739":180}"> </span></p> <p><strong><span data-contrast="none">Position Summary</span></strong><span data-ccp-props="{"335559738":320,"335559739":140}"> </span></p> <p><span data-contrast="auto">We are seeking a highly motivated and exceptionally organized Research Associate II to expand our protein production capabilities across two complementary scales: bulk expression and purification of individual high-priority targets, and high-throughput parallel production of large variant panels. This hands-on, laboratory-facing role will execute and advance scalable protein expression, purification, normalization, and characterization workflows that feed the design–build–test cycles at the core of our platform.</span><span data-ccp-props="{"335559739":180}"> </span></p> <p><span data-contrast="auto">The successful candidate will bring direct, practical experience in recombinant protein production and chromatographic purification at both preparative and high-throughput scale — together with the ability to execute experiments, run high-throughput analytics, troubleshoot workflows, and deliver well-characterized protein reagents on rapid timelines. This individual will work closely with Protein Engineering, Chemistry, and Assay Development team to align production activities with program priorities and experimental timelines. Meticulous record-keeping and sample traceability are essential to this role. Experience with liquid handlers, magnetic-bead purification platforms such as the KingFisher, cell-free expression, or workflow scripting is preferred.</span><span data-ccp-props="{"335559739":180}"> </span></p> <p><span data-contrast="auto">The ideal candidate thrives in a fast-paced, collaborative environment, enjoys solving technical challenges, and is motivated to apply automation and emerging technologies to developing a synthetic biology platform. </span><span data-ccp-props="{"335559739":180}"> </span></p> <p><strong><span data-contrast="none">Key Responsibilities</span></strong><span data-ccp-props="{"335559738":320,"335559739":140}"> </span></p> <p><strong><span data-contrast="auto">Bulk Expression & Purification</span></strong><span data-ccp-props="{"335559738":200,"335559739":100}"> </span></p> <ul> <li data-leveltext="•" data-font="Calibri" data-listid="1" data-list-defn-props="{"335552541":1,"335559685":431,"335559991":244,"469769242":[8226],"469777803":"left","469777804":"•","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="1"><span data-contrast="auto">Execute multi-liter recombinant expression campaigns in bacteria from construct transformation and culture growth through induction, harvest, and lysis.</span><span data-ccp-props="{"335559739":100}"> </span></li> </ul> <ul> <li data-leveltext="•" data-font="Calibri" data-listid="1" data-list-defn-props="{"335552541":1,"335559685":431,"335559991":244,"469769242":[8226],"469777803":"left","469777804":"•","469777815":"hybridMultilevel"}" data-aria-posinset="2" data-aria-level="1"><span data-contrast="auto">Design, build, and run preparative purification methods on ÄKTA FPLC systems using UNICORN, including affinity, ion exchange, size exclusion, and mixed-mode chromatography.</span><span data-ccp-props="{"335559739":100}"> </span></li> </ul> <ul> <li data-leveltext="•" data-font="Calibri" data-listid="1" data-list-defn-props="{"335552541":1,"335559685":431,"335559991":244,"469769242":[8226],"469777803":"left","469777804":"•","469777815":"hybridMultilevel"}" data-aria-posinset="3" data-aria-level="1"><span data-contrast="auto">Deliver milligram-to-gram quantities of highly pure, well-characterized protein.</span><span data-ccp-props="{"335559739":100}"> </span></li> </ul> <ul> <li data-leveltext="•" data-font="Calibri" data-listid="1" data-list-defn-props="{"335552541":1,"335559685":431,"335559991":244,"469769242":[8226],"469777803":"left","469777804":"•","469777815":"hybridMultilevel"}" data-aria-posinset="4" data-aria-level="1"><span data-contrast="auto">Troubleshoot expression and purification challenges such as low yield, insolubility, aggregation, and proteolysis, and drive protocol improvements that increase yield and purity.</span><span data-ccp-props="{"335559739":100}"> </span></li> </ul> <p><strong><span data-contrast="auto">High-Throughput Production & Analytics</span></strong><span data-ccp-props="{"335559738":200,"335559739":100}"> </span></p> <ul> <li data-leveltext="•" data-font="Calibri" data-listid="1" data-list-defn-props="{"335552541":1,"335559685":431,"335559991":244,"469769242":[8226],"469777803":"left","469777804":"•","469777815":"hybridMultilevel"}" data-aria-posinset="5" data-aria-level="1"><span data-co